rabbit primary monoclonal antibodies against α sma  (Proteintech)

Journal: Chinese Medicine

Article Title: Combination of mangiferin and T0901317 targeting autophagy promotes cholesterol efflux from macrophage foam cell in atherosclerosis

doi: 10.1186/s13020-023-00876-9

Figure Lengend Snippet: Effects of mangiferin (MGF) and T0901317 (T0) on inhibition of AS progression. Apoe −/− mice fed with high fat diet (HFD) in four groups (n = 10/group) received the following treatment for 16 weeks. Ctrl: vehicle; T0: oral gavage of T0 (1 mg∙kg −1 bodyweight/day); MGF: oral gavage of MGF (200 mg kg −1 bodyweight/day); T0 + MGF: oral gavage of T0 and MGF. After treatment, aortas were collected for the following assays. A Lesions in en face aorta were determined by Oil Red O staining and lesion areas were expressed as % of total en face aorta areas. Scale bar, 5 mm. *** p < 0.001, ** p < 0.01, * p < 0.05, NS: not significant (n = 10). B Sinus lesions in aortic root were determined by Oil Red O staining and lesion areas were expressed as μm 2 /section. Scale bar, 400 μm. * p < 0.05 (n = 9). C Collagen content areas were determined by Masson staining of aortic root cross sections and quantified by a computer-assisted image analysis protocol (n = 3). Scale bar, 100 μm. D Expression of α-SMA (stained with red fluorescent color) was determined by immunofluorescent staining of aortic root cross sections and quantified by a computer-assisted image analysis protocol. Scale bar, 100 μm. *** p < 0.001, ** p < 0.01, * p < 0.05 (n = 3)

Article Snippet: Primary rabbit polyclonal antibodies against α-SMA (14395), CD36 (18836), Beclin1 (11306), ATG5 (10181), and ATG7 (10088) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Inhibition, Staining, Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner

doi: 10.1155/2020/2452848

Figure Lengend Snippet: The sequences of the primers for real-time PCR.

Article Snippet: Primary antibodies against anti-rabbit α -SMA (14395-1-AP), α 1(I) procollagen (14395-1-AP), fibronectin (15613-1-AP), collagen I (14695-1-AP), and GAPDH (13937-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner

doi: 10.1155/2020/2452848

Figure Lengend Snippet: Effect of WFA on PDGF-BB-induced liver fibrosis and the expression of SIRT3 in activated JS1 cells. JS1 cells were treated with DMSO (0.02%, w / v ), PDGF-BB (20 ng/ml), and the indicated concentrations of WFA for 24 h. (a–c) Quantification of α -SMA, fibronectin, and α 1(I) procollagen expression in JS1 cells were carried out by real-time PCR and Western blot. (d) Quantification of SIR2 family (SIRT1-SIRT7) mRNA expression was assessed by real-time PCR. (e) The effect of WFA on the enzyme activity of SIRT3 deacetylase in activated JS1 cells. (f) Quantification of SIRT3 protein expression was assessed by Western blot in activated JS1 cells. For the statistics of each panel in this figure, data are expressed as mean ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Primary antibodies against anti-rabbit α -SMA (14395-1-AP), α 1(I) procollagen (14395-1-AP), fibronectin (15613-1-AP), collagen I (14695-1-AP), and GAPDH (13937-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Activity Assay, Histone Deacetylase Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner

doi: 10.1155/2020/2452848

Figure Lengend Snippet: Effect of SIRT3 depletion on the antifibrogenic effect of WFA in activated JS1 cells. (a) After SIRT3 siRNA or NC siRNA was transfected into JS1 cells for 24 h, SIRT3 protein expression was measured with Western blots. (b) After SIRT3 siRNA or NC siRNA was transfected into JS1 cells for 24 h, cells were treated with DMSO (0.02%, w / v ), PDGF-BB (20 ng/ml), and the indicated concentrations of WFA for 24 h. SIRT3 protein expression was measured by Western blots. (c–e) Quantification of α -SMA, fibronectin, and α 1(I) procollagen expression in SIRT3-siRNA-treated or untreated JS1 cells (with NC siRNA transfection). For the statistics of each panel in this figure, data are expressed as mean ± SD ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Primary antibodies against anti-rabbit α -SMA (14395-1-AP), α 1(I) procollagen (14395-1-AP), fibronectin (15613-1-AP), collagen I (14695-1-AP), and GAPDH (13937-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Transfection, Expressing, Western Blot

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner

doi: 10.1155/2020/2452848

Figure Lengend Snippet: Effect of WFA on the CCl 4 -induced collagen production, liver fibrosis, and SIRT3 expression in mice. (a) Liver sections were stained with Masson's trichrome and picrosirius red reagents, and representative photographs are shown (scale bar: 100 μ m), respectively. (b) Measurement of hydroxyproline levels in liver homogenate, ∗ p < 0.05. (c) Real-time PCR analyses of SIRT3, α -SMA, fibronectin, and α 1(I) procollagen in liver tissues. GAPDH was used as the invariant control. (d) Western blot analyses of liver proteins with densitometry. Representative blots were from three independent experiments. (e) Quantified western blot results of liver proteins. For the statistics of each panel in this figure, data are expressed as mean ± SD ( n = 3). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus the control group; # p < 0.05 and ## p < 0.01 versus the model (CCl 4 ) group.

Article Snippet: Primary antibodies against anti-rabbit α -SMA (14395-1-AP), α 1(I) procollagen (14395-1-AP), fibronectin (15613-1-AP), collagen I (14695-1-AP), and GAPDH (13937-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Expressing, Staining, Real-time Polymerase Chain Reaction, Control, Western Blot

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner

doi: 10.1155/2020/2452848

Figure Lengend Snippet: Effect of WFA on the CCl 4 -induced liver fibrosis in WT and SIRT3 KO mice. (a–c) The serum levels of HA, LN, and PC-III were examined in both WT and SIRT3 KO mice. (d–f) Real-time PCR analyses of α -SMA, fibronectin, and α 1(I) procollagen in liver tissues. GAPDH was used as the invariant control. (g) Western blot analyses of liver proteins with densitometry. Representative blots were from three independent experiments. (h–j) Quantified western blot results of liver proteins. For the statistics of each panel in this figure, data are expressed as mean ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Primary antibodies against anti-rabbit α -SMA (14395-1-AP), α 1(I) procollagen (14395-1-AP), fibronectin (15613-1-AP), collagen I (14695-1-AP), and GAPDH (13937-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Real-time Polymerase Chain Reaction, Control, Western Blot